PhD in Transcriptomic Research
Job No:
G113
Location:
Darlinghurst, Sydney
PhD in Transcriptomic Research
Project: Discovering gene fusions in cancer
Supervisor: Dr Tim Mercer
Co-supervisor: Dr Jim Blackburn
The transcriptome is defined as the assortment of all RNA molecules present within a cell or tissue. Variations in transcriptome composition, generated through differential gene expression and splicing, reflects changes in cellular states and cell differentiation. As such, change in transcriptome content can also be indicative of the onset of disease. Lead by Dr Tim Mercer, the key focus of the Transcriptomic Research laboratory is the study of how this information is encoded, organised, expressed and evolves within the human genome. Our team recently developed a targeted high resolution RNA sequencing strategy, termed Capture Sequencing. The technique enables more sensitive gene discovery, more precise measurement of transcript abundance, and the dissection of aberrations such as gene fusion.
Fusion genes occur when a chromosomal break joins two different loci. Gene fusion accounts for a high proportion of cancers, and accurate molecular diagnosis is crucial for effective treatment and management of the disease. Using the Capture Sequencing technology, we have developed a single test capable of identifying the presence of gene fusions – including previously known and novel rearrangements.
We are seeking a talented PhD student to join our research team to investigate gene fusion in cancer. The project will involve implementing the Capture Sequencing test in a large patient cohort and analysing the sequencing data for fusion status. The project will employ a variety of techniques, including (but not restricted to) tissue culture, molecular biology, next generation sequencing technologies, and bioinformatics. A demonstrated background in any of these techniques is highly desirable.
For further details on the project, please contact Dr Jim Blackburn (j.blackburn@garvan.org.au) or Dr Tim Mercer (t.mercer@garvan.org.au